• Detailed description of the image analysis workflow, including machine learning model training dataset, for the article: "Phosphate starvation decouples cell differentiation from DNA replication control in the dimorphic bacterium Caulobacter crescentus", Hallgren et al. (2023; PLOS Genetics).

This dataset contains a detailed description of the image analysis procedure used in Hallgren et al. (2023; PLOS Genetics) to perform single-cell measurements of the bacterium Caulobacter crescentus. More specifically, t...

• Whole-genome sequencing of follicular thyroid carcinomas reveal recurrent mutations in microRNA processing subunit DGCR8

Background The genomic and transcriptomic landscape of widely invasive follicular thyroid carcinomas (wiFTCs) is poorly characterized, and a large subset of these tumours lack information on credible genetic driver event...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Immunocytofluoroscense of Hsp70-mutant yeast strains with primary antibodies against Hsp42p

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Western blots of SDS-PAGE gels with primary antibodies against Hsp70p and Pgk1p

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Hsp70-mutant yeast strains with the misfolding marker protein guk1-7-GFP and with or without an intact HSP104-allele, imaged during mid-exponential growth

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Intracellular colocalisation of the chaperone Ssa4-GFP and the metacaspase Mca1-RFP before and after heat stress in a Hsp70-mutant yeast cell strain

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - The molecular chaperone GFP-Hsp104 before and after heat stress in a Hsp70-mutant yeast strain with exogenous complementation of wildtype and chimaeric mutant alleles of yeast Hsp70-alleles

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - The misfolding protein marker guk1-7-GFP before, during and after in recovery from heat shock stress in Hsp70-mutant yeast strains

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...

• Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Hsp70-mutant yeast cells with the misfolding marker protein guk1-7-GFP, the nucleolar marker Sik1-RFP and nuclear staining with DAPI imaged before, directly after, and during recovery from heat stress

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate...